Composite

Part:BBa_K4501015:Design

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-22)


CD63_His-tag L7Ae (x2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2192
    Illegal XhoI site found at 1760
    Illegal XhoI site found at 2528
    Illegal XhoI site found at 2534
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2309
    Illegal NgoMIV site found at 2791
    Illegal NgoMIV site found at 3650
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We split the part into 4 different fragments to be assembled by Golden Gate. To incorporate the 2nd unit of L7Ae, classical restriction cloning is to be performed. L7Ae from the BBa_K4501014 part is to be amplified using primers with restriction recognition sites at the sides.


Source

Designed by oursleves.

References